NitrAdine® Core Technology



What is bonyf’s core technology NitrAdine®?

NitrAdine® is a clinically proven, non-antibiotic formulation with disinfecting and pain reducing properties. It is the basis of multiple products manufactured and commercialized by bonyf. The NitrAdine® formulation has vast potential for additional product development.

Research and development are ongoing in the oral, wound care and general medical equipment disinfection areas (including veterinary) for both consumer and professional use.

Why was NitrAdine® developed?

NitrAdine® is bonyf’s proprietary formulation that was originally developed in 2001 to disinfect removable dentures that become quickly contaminated with pathogenic micro-organisms such as Candida albicans. 65% of people wearing dentures experience burning sensations, mucosal inflammation and discomfort in the mouth due to their contaminated denture. To ease this discomfort NitrAdine®-based cleaning and disinfecting effervescent tablets for dentures were developed.

As NitrAdine® removes Candida albicans and other pathogenic micro-organisms from both the surface and the microporosities of dentures successfully, additional NitrAdine® containing products were developed for disinfecting other removable oral appliances. These appliances include orthodontics, snoring and bruxism devices.

What makes the NitrAdine® formulation unique?

NitrAdine®’s uniqueness is due to its high efficacy in removing pathogenic biofilm from removable dentures, orthodontic appliances, toothbrushes, teeth, gums and dental implants. Multiple clinical studies have demonstrated its efficacy. It is safe to use and does not induce any side effects. By using NitrAdine® any potential tissue inflammation and pain caused by the presence of biofilm is significantly reduced, therefore restoring healthy mouth and gums.

NitrAdine® is not an antibiotic drug, nor a disinfecting molecule such as chlorhexidine, but a formulation of 9 chemical ingredients that interact resulting in its disinfecting and pain reducing properties.

Chemical Structures of

NitrAdine® ingredients

What is the mode of action of the NitrAdine® formulation?

The mode of action of NitrAdine®’s efficacy is based on the combined action of the formulation’s surfactant ingredient (Sodium lauryl sulphate) and the other ingredients. When NitrAdine® is dissolved in water (tablet formulation) or comes into contact with moisture (paste formulation), the surfactant disrupts the biofilm membrane proteins.

The interaction of the other ingredients results in a slow release of a non-toxic concentration of approx. 0.02% hypochlorite, killing micro-organisms (bacteria, yeast and viruses) present in the biofilm.

What does NitrAdine® do?

The NitrAdine® formulation has proven bactericidal, yeasticidal and virucidal properties and in addition induces a significant reduction of biofilm formed by a variety of disease inducing micro-organisms to clinically insignificant levels.

These include biofilms formed by (i) bacteria (Staphylococcus aureus, Streptococcus mutans, Pseudomonas aeruginosa, Porphyromonas gingivalis, Provotella intermedia, Aggregatibacter actinomycetemcomitans); (ii) yeasts (Candida species); and (iii) viruses (Herpes simplex, Human Papilloma virus, Adenovirus, Poliovirus, Murine norovirus and all enveloped viruses (including Coronavirus).

Several independent academic pre-clinical and clinical studies have demonstrated NitrAdine®’s biofilm removal properties, consequently shifting any painful biofilm-induced tissue inflammation into a healthy healing state.

What are biofilms and why should they be eliminated?

Biofilms are a collection of micro-organisms that are enclosed in a self-produced three-dimensional polysaccharide matrix that attaches and grows on many different surfaces.

Biofilms have antimicrobial drug resistance features and can be found on dentures, orthodontic appliances, teeth, gums, dental implants but also on toothbrushes.

It is necessary to eliminate biofilms as they will generate microbial and chronic infections in the body, which can lead to a number of human diseases. In addition, biofilms can attach to the surface of skin wounds, causing chronic inflammation and therefore delaying and/or impairing wound healing.

What are the current and future applications using the NitrAdine® formulation?


products produced by bonyf highlighting bonyf’s core technology

Current developed applications of NitrAdine® have resulted in unique products already manufactured by bonyf for denture related stomatitis (NitrAdine® Denture Disinfecting Effervescent Tabs), dental orthodontics (NitrAdine® Ortho-Junior Disinfecting Effervescent Tabs), bleeding gums (PerioTabs®), implant related diseases (PerioTabs®), swollen gums (PerioTabs®), mouth ulcers (AphtoFix®), and cracks on heels and fingers (HeelFix and FingerFix).

Additional NitrAdine® based products are part of bonyf’s future short- and long-term developments. These include an anti-biofilm toothpaste and mouthwash, products for medical equipment disinfection, for pre-implant surgery disinfection, impaired wound healing, wart removal, cold sore and bed sore healing, as well as bleeding gums in pets.